Multiplex PCR detection of Escherichia coli carrying Shiga toxin genes in E. coli isolated from patients with diarrhea in Hajar hospital, Shahrekord, Iran
Jentashapir Journal of Health Research: 4 (3); 193-202
June 30, 2013
Article Type: Research Article
January 1, 1970
March 15, 2012
Multiplex PCR detection of Escherichia coli carrying Shiga toxin genes in E. coli isolated from patients with diarrhea in Hajar hospital, Shahrekord, Iran,
Jentashapir J Health Res.
Online ahead of Print
Diarrhea causes 4% of all deaths and 5% of health loss lead to disability world-wide. Shiga-toxin-producing Escherichia coli can cause mild watery diarrhea to more serious complications from hemorrhagic colitis, and hemolytic uremic syndrome to even death. Present study was conducted to detect E. coli carrying Shiga toxin genes (stx1 and stx2) in E. coli isolated from patients with diarrhea in Hajar Hospital, Shahrekord, Iran.
Material and methods:
A total of 110 E. coli isolates collected from patients with diarrhea in 2011 were evaluated to investigate stx1, stx2 and eaeA genes by multiplex PCR.
The rate of stx1 and eaeA-positive E. coli isolates were 2.7% (3/110) and 1.8% (2/110), respectively. However, stx2 gene was not found in any isolate, eaeA was not also present in stx1-positive E. coli isolates. Virulence Genes were not found in E. coli isolated from Women. The most antibiotic resistance rates of isolated virulence genes-positive E. coli were to Ampicillin and Trimethoprim and lowest antibiotic resistance was observed against Gentamicin and Chloramphenicol.
© 2013, Jentashapir Journal of Health Research. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.