Jentashapir Journal of Health Research

Published by: Kowsar

Assessment of the Separation of X- and Y-Chromosome Bearing Sperms of Fertile Men by Albumin Gradients Using Real Time PCR

Sahar Baheri 1 , Ghasem Saki 1 , * , Javad Mohammadi Asl 2 and Ali Khodadadi 3
Authors Information
1 Physiology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
2 Department of Genetics, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
3 Department of Immunology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
Article information
  • Jentashapir Journal of Health Research: April 01, 2016, 7 (2); e31079
  • Published Online: April 19, 2016
  • Article Type: Research Article
  • Received: June 27, 2015
  • Accepted: August 26, 2015
  • DOI: 10.17795/jjhr-31079

To Cite: Baheri S, Saki G, Mohammadi Asl J, Khodadadi A. Assessment of the Separation of X- and Y-Chromosome Bearing Sperms of Fertile Men by Albumin Gradients Using Real Time PCR, Jentashapir J Health Res. 2016 ; 7(2):e31079. doi: 10.17795/jjhr-31079.

Copyright © 2016, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Patients and Methods
4. Results
5. Discussion
  • 1. Stolkowski J, Lorrain J. Preconceptional selection of fetal sex. Int J Gynaecol Obstet. 1980; 18(6): 440-3[PubMed]
  • 2. Holt WV, O'Brien J, Abaigar T. Applications and interpretation of computer-assisted sperm analyses and sperm sorting methods in assisted breeding and comparative research. Reprod Fertil Dev. 2007; 19(6): 709[DOI]
  • 3. Schulman JD, Karabinus DS. Scientific aspects of preconception gender selection. Reprod Biomed Online. 2005; 10 Suppl 1: 111-5[PubMed]
  • 4. Ericsson RJ, Langevin CN, Nishino M. Isolation of fractions rich in human Y sperm. Nature. 1973; 246(5433): 421-4[PubMed]
  • 5. Rose GA, Wong A. Experiences in Hong Kong with the theory and practice of the albumin column method of sperm separation for sex selection. Hum Reprod. 1998; 13(1): 146-9[PubMed]
  • 6. Jacobsen R, Moller H, Engholm G. Fertility rates in Denmark in relation to the sexes of preceding children in the family. Hum Reprod. 1999; 14(4): 1127-30[PubMed]
  • 7. Vidal F, Moragas M, Catala V, Torello MJ, Santalo J, Calderon G, et al. Sephadex filtration and human serum albumin gradients do not select spermatozoa by sex chromosome: a fluorescent in-situ hybridization study. Hum Reprod. 1993; 8(10): 1740-3[PubMed]
  • 8. Missmer SA, Jain T. Preimplantation sex selection demand and preferences among infertility patients in Midwestern United States. J Assist Reprod Genet. 2007; 24(10): 451-7[DOI][PubMed]
  • 9. Cran DG. XY sperm separation and use in artificial insemination and other ARTs. Soc Reprod Fertil Suppl. 2007; 65: 475-91[PubMed]
  • 10. Pyrzak R. Separation of X- and Y-bearing human spermatozoa using albumin gradients. Hum Reprod. 1994; 9(10): 1788-90[PubMed]
  • 11. Pyrzak R, Garrison CP. Application of DNA in situ hybridization using biotinylated probes of human X and Y sperm. J Androl. 1990; 11: 46
  • 12. Beernink FJ, Dmowski WP, Ericsson RJ. Sex preselection through albumin separation of sperm. Fertil Steril. 1993; 59(2): 382-6[PubMed]
  • 13. Wang HX, Flaherty SP, Swann NJ, Matthews CD. Assessment of the separation of X- and Y-bearing sperm on albumin gradients using double-label fluorescence in situ hybridization. Fertil Steril. 1994; 61(4): 720-6[PubMed]
  • 14. Flaherty SP, Michalowska J, Swann NJ, Dmowski WP, Matthews CD, Aitken RJ. Albumin gradients do not enrich Y-bearing human spermatozoa. Hum Reprod. 1997; 12(5): 938-42[PubMed]
  • 15. Henrion R, Papiernik E, Stolkowski J. [Choice of sex before conception?]. Concours Med. 1975; 97(43): 7077-8[PubMed]
  • 16. Stolkowski J, Choukroun J. Preconception selection of sex in man. Isr J Med Sci. 1981; 17(11): 1061-7[PubMed]
  • 17. Esfahani N, Kalantari A. To achieve the desired sex in fertile patients and infertile women undergoing intrauterine sperm injection treatment albumin method. JIMU. 2003; 21: 24-8
  • 18. Jaffe SB, Jewelewicz R, Wahl E, Khatamee MA. A controlled study for gender selection. Fertil Steril. 1991; 56(2): 254-8[PubMed]
  • 19. Silverman AY, Stephens SR, Drouin MT, Zack RG, Osborne J, Ericsson SA. Female sex selection using clomiphene citrate and albumin separation of human sperm. Hum Reprod. 2002; 17(5): 1254-6[PubMed]
  • 20. Khalili MA, Khani B, Baghazadeh S, Tabibnejad N. Sex selection by using albumin gradient technique for sperm separation in IUI cycles. JRI. 2007; 8(3)
  • 21. Corson SL, Batzer FR, Alexander NJ, Schlaff S, Otis C. Sex selection by sperm separation and insemination. Fertil Steril. 1984; 42(5): 756-60[PubMed]
  • 22. Check JH, Shanis BS, Cooper SO, Bollendorf A. Male sex preselection: swim-up technique and insemination of women after ovulation induction. Arch Androl. 1989; 23(2): 165-6[PubMed]
  • 23. Johnson LA, Welch GR, Keyvanfar K, Dorfmann A, Fugger EF, Schulman JD. Gender preselection in humans? Flow cytometric separation of x and y spermatozoa for the prevention of x-linked diseases. Obstet Gynecol Surv. 1994; 49(5): 342-3[DOI]
  • 24. Kahraman S, Bahce M, Samli H, Imirzalioglu N, Yakisn K, Cengiz G, et al. Healthy births and ongoing pregnancies obtained by preimplantation genetic diagnosis in patients with advanced maternal age and recurrent implantation failure. Hum Reprod. 2000; 15(9): 2003-7[PubMed]
  • 25. Platteau P, Sermon K, Seneca S, Van Steirteghem A, Devroey P, Liebaers I. Preimplantation genetic diagnosis for fragile Xa syndrome: difficult but not impossible. Hum Reprod. 2002; 17(11): 2807-12[PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments