Jentashapir Journal of Health Research

Published by: Kowsar

Melatonin Effect on Immature Mouse Testicular Tissues, Vitrified-Thawed With Different Cryoprotectant Media

Mohammadreza Gholami 1 , Ghasem Saki 2 , * , Masoud Hemadi 3 , Ali Khodadadi 4 and Javad Mohammadiasl 5
Authors Information
1 Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, IR Iran
2 Physiology Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
3 Fertility, Infertility and Perinatology Research Center, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
4 Department of Immunology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
5 Department of Medical Genetics, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
Article information
  • Jentashapir Journal of Health Research: June 01, 2015, 6 (3); e28704
  • Published Online: June 27, 2015
  • Article Type: Research Article
  • Received: June 16, 2014
  • Accepted: December 26, 2014
  • DOI: 10.5812/jjhr.28704v2

To Cite: Gholami M, Saki G, Hemadi M, Khodadadi A, Mohammadiasl J. Melatonin Effect on Immature Mouse Testicular Tissues, Vitrified-Thawed With Different Cryoprotectant Media, Jentashapir J Health Res. 2015 ; 6(3):e28704. doi: 10.5812/jjhr.28704v2.

Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. van den Berg H, Furstner F, van den Bos C, Behrendt H. Decreasing the number of MOPP courses reduces gonadal damage in survivors of childhood Hodgkin disease. Pediatr Blood Cancer. 2004; 42(3): 210-5[DOI][PubMed]
  • 2. Lee SJ, Schover LR, Partridge AH, Patrizio P, Wallace WH, Hagerty K, et al. American Society of Clinical Oncology recommendations on fertility preservation in cancer patients. J Clin Oncol. 2006; 24(18): 2917-31[DOI][PubMed]
  • 3. Mitchell RT, Saunders PT, Sharpe RM, Kelnar CJ, Wallace WH. Male fertility and strategies for fertility preservation following childhood cancer treatment. Endocr Dev. 2009; 15: 101-34[DOI][PubMed]
  • 4. Wallace WHB, Anderson RA, Irvine DS. Fertility preservation for young patients with cancer: who is at risk and what can be offered? Lancet Oncol. 2005; 6(4): 209-18
  • 5. Green DM, Kawashima T, Stovall M, Leisenring W, Sklar CA, Mertens AC, et al. Fertility of male survivors of childhood cancer: a report from the Childhood Cancer Survivor Study. J Clin Oncol. 2010; 28(2): 332-9[DOI][PubMed]
  • 6. Orwig KE, Schlatt S. Cryopreservation and transplantation of spermatogonia and testicular tissue for preservation of male fertility. J Natl Cancer Inst Monogr. 2005; (34): 51-6[DOI][PubMed]
  • 7. Mazur P. Equilibrium, quasi-equilibrium, and nonequilibrium freezing of mammalian embryos. Cell Biophys. 1990; 17(1): 53-92[PubMed]
  • 8. Curaba M, Verleysen M, Amorim CA, Dolmans MM, Van Langendonckt A, Hovatta O, et al. Cryopreservation of prepubertal mouse testicular tissue by vitrification. Fertil Steril. 2011; 95(4): 1229-34 e1[DOI][PubMed]
  • 9. Curaba M, Poels J, van Langendonckt A, Donnez J, Wyns C. Can prepubertal human testicular tissue be cryopreserved by vitrification? Fertil Steril. 2011; 95(6): 2123 e9-12[DOI][PubMed]
  • 10. Ahmad R, Haldar C. Effect of intra-testicular melatonin injection on testicular functions, local and general immunity of a tropical rodent Funambulus pennanti. Endocrine. 2010; 37(3): 479-88[DOI][PubMed]
  • 11. Harms C. Melatonin is protective in necrotic but not in caspase-dependent, free radical-independent apoptotic neuronal cell death in primary neuronal cultures. FASEB J. 2000; 14(12): 1814-24
  • 12. Succu S, Berlinguer F, Pasciu V, Satta V, Leoni GG, Naitana S. Melatonin protects ram spermatozoa from cryopreservation injuries in a dose-dependent manner. J Pineal Res. 2011; 50(3): 310-8[PubMed]
  • 13. Dominguez-Rebolledo AE, Fernandez-Santos MR, Bisbal A, Ros-Santaella JL, Ramon M, Carmona M, et al. Improving the effect of incubation and oxidative stress on thawed spermatozoa from red deer by using different antioxidant treatments. Reprod Fertil Dev. 2010; 22(5): 856-70[PubMed]
  • 14. Gholami M, Saki G, Hemadi M, Khodadadi A, Mohammadi-asl J. Supplementation vitrified-thawed media with melatonin do not protecting immature mouse testicular tissue from vitrified-thawed induced injury. Asian J Anim Vet Adv. 2012; 7: 940-9
  • 15. Fildes JE, Yonan N, Keevil BG. Melatonin-a pleiotropic molecule involved in pathophysiological processes following organ transplantation. Immunology. 2009; 127(4): 443-9[PubMed]
  • 16. Hardeland R, Cardinali DP, Srinivasan V, Spence DW, Brown GM, Pandi-Perumal SR. Melatonin--a pleiotropic, orchestrating regulator molecule. Prog Neurobiol. 2011; 93(3): 350-84[DOI][PubMed]
  • 17. Zawilska JB, Skene DJ, Arendt J. Physiology and pharmacology of melatonin in relation to biological rhythms. Pharmacol Rep. 2009; 61(3): 383-410[PubMed]
  • 18. Hemadi M, Abolhassani F, Akbari M, Sobhani A, Pasbakhsh P, Ahrlund-Richter L, et al. Melatonin promotes the cumulus-oocyte complexes quality of vitrified-thawed murine ovaries; with increased mean number of follicles survival and ovary size following heterotopic transplantation. Eur J Pharmacol. 2009; 618(1-3): 84-90[DOI][PubMed]
  • 19. Gholami M, Saki G, Hemadi M, Khodadadi A, Mohammadi-asl J. Melatonin improves spermatogonial stem cells transplantation efficiency in azoospermic mice. Iran J Basic Med Sci. 2014; 17(2): 93-9
  • 20. Hemadi M, Zargar M, Sobhani A, Sobhani A. Assessment of morphological and functional changes in neonate vitrified testis grafts after host treatment with melatonin. Folia Morphol (Warsz). 2011; 70(2): 95-102[PubMed]
  • 21. Ahmad R, Haldar C. Melatonin and androgen receptor expression interplay modulates cell-mediated immunity in tropical rodent Funambulus pennanti: an in-vivo and in-vitro study. Scand J Immunol. 2010; 71(6): 420-30[PubMed]
  • 22. da Silva CM, Macias-Garcia B, Miro-Moran A, Gonzalez-Fernandez L, Morillo-Rodriguez A, Ortega-Ferrusola C, et al. Melatonin reduces lipid peroxidation and apoptotic-like changes in stallion spermatozoa. J Pineal Res. 2011; 51(2): 172-9[PubMed]
  • 23. Martin-Renedo J, Mauriz JL, Jorquera F, Ruiz-Andres O, Gonzalez P, Gonzalez-Gallego J. Melatonin induces cell cycle arrest and apoptosis in hepatocarcinoma HepG2 cell line. J Pineal Res. 2008; 45(4): 532-40[PubMed]
  • 24. Millan-Plano S, Piedrafita E, Miana-Mena FJ, Fuentes-Broto L, Martinez-Ballarin E, Lopez-Pingarron L, et al. Melatonin and structurally-related compounds protect synaptosomal membranes from free radical damage. Int J Mol Sci. 2010; 11(1): 312-28[DOI][PubMed]
  • 25. Radogna F, Cristofanon S, Paternoster L, D'Alessio M, De Nicola M, Cerella C, et al. Melatonin antagonizes the intrinsic pathway of apoptosis via mitochondrial targeting of Bcl-2. J Pineal Res. 2008; 44(3): 316-25[PubMed]
  • 26. Radogna F, Paternoster L, Albertini MC, Accorsi A, Cerella C, D'Alessio M, et al. Melatonin as an apoptosis antagonist. Ann N Y Acad Sci. 2006; 1090: 226-33[DOI][PubMed]
  • 27. Radogna F, Paternoster L, Albertini MC, Cerella C, Accorsi A, Bucchini A, et al. Melatonin antagonizes apoptosis via receptor interaction in U937 monocytic cells. J Pineal Res. 2007; 43(2): 154-62[PubMed]
  • 28. Reiter RJ, Tan DX, Manchester LC, Paredes SD, Mayo JC, Sainz RM. Melatonin and reproduction revisited. Biol Reprod. 2009; 81(3): 445-56[DOI][PubMed]
  • 29. Zhao Y, Qi LW, Wang WM, Saxena PK, Liu CZ. Melatonin improves the survival of cryopreserved callus of Rhodiola crenulata. J Pineal Res. 2011; 50(1): 83-8[PubMed]
  • 30. Milazzo JP, Vaudreuil L, Cauliez B, Gruel E, Masse L, Mousset-Simeon N, et al. Comparison of conditions for cryopreservation of testicular tissue from immature mice. Hum Reprod. 2008; 23(1): 17-28[DOI][PubMed]
  • 31. Gholami M, Saki G, Hemadi M, Khodadadi A, Mohamma-di-Asl J. Effect of Melatonin on the Expression of Apoptotic Genes in Vitrified-thawed Spermatogonia Stem Cells Type A of 6-Day-Old Mice. Iran J Basic Med Sci. 2013; 16(8): 906-9[PubMed]
  • 32. Gholami M, Hemadi M, Saki G, Zendedel A, Khodadadi A, Mohammadi-Asl J. Does prepubertal testicular tissue vitrification influence spermatogonial stem cells (SSCs) viability? J Assist Reprod Genet. 2013; 30(10): 1271-7[PubMed]
  • 33. Sanmartin-Suarez C, Soto-Otero R, Sanchez-Sellero I, Mendez-Alvarez E. Antioxidant properties of dimethyl sulfoxide and its viability as a solvent in the evaluation of neuroprotective antioxidants. J Pharmacol Toxicol Methods. 2011; 63(2): 209-15[DOI][PubMed]
  • 34. Balci D, Can A. The assessment of cryopreservation conditions for human umbilical cord stroma-derived mesenchymal stem cells towards a potential use for stem cell banking. Curr Stem Cell Res Ther. 2013; 8(1): 60-72[PubMed]
  • 35. Uchendu EE, Leonard SW, Traber MG, Reed BM. Vitamins C and E improve regrowth and reduce lipid peroxidation of blackberry shoot tips following cryopreservation. Plant Cell Rep. 2010; 29(1): 25-35[DOI][PubMed]
  • 36. Succu S, Pasciu V, Manca ME, Chelucci S, Torres-Rovira L, Leoni GG, et al. Dose-dependent effect of melatonin on postwarming development of vitrified ovine embryos. Theriogenology. 2014; 81(8): 1058-66[DOI][PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments