Jentashapir Journal of Health Research

Published by: Kowsar

Wound Healing Effect of an Ointment Made From a Mixture of Brassica oleracea var, Punica granatum, and Plantago major L Extracts in Rats

Mohammad Hassan Bazafkan 1 , Ameneh Hardani 2 , * , Mohammad Reza Afzal Zadeh 3 , Ashraf Amir Zargar 4 , Mahmoud Orazi Zadeh 5 , Ali Asghar Hemmati 6 , Esrafil Mansori 5 , Majid Asadi-Samani 7 , Maryam Ghasemiboroon 2 and Vesam Kooti 8
Authors Information
1 Department of Environmental Health Engineering, School of Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
2 School of Health, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
3 School of Veterinary Medicine, Shahid Chamran University, Ahvaz, IR Iran
4 Department of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
5 Department of Anatomical Sciences, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
6 Department of Pharmacology and Toxicology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
7 Medical Plant Research Center, Shahrekord University of Medical Sciences, Shahrekord, IR Iran
8 Department of Laboratory Sciences, School of Paramedicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IR Iran
Article information
  • Jentashapir Journal of Health Research: August 01, 2014, 5 (4); e21877
  • Published Online: August 10, 2014
  • Article Type: Research Article
  • Received: March 3, 2013
  • Revised: March 9, 2013
  • Accepted: October 7, 2013
  • DOI: 10.17795/jjhr-21877

To Cite: Bazafkan M H, Hardani A, Afzal Zadeh M R, Amir Zargar A, Orazi Zadeh M, et al. Wound Healing Effect of an Ointment Made From a Mixture of Brassica oleracea var, Punica granatum, and Plantago major L Extracts in Rats, Jentashapir J Health Res. 2014 ; 5(4):e21877. doi: 10.17795/jjhr-21877.

Copyright © 2014, Ahvaz Jundishapur University of Medical Sciences. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Oz AT. Effects of harvest date and conditions of storage of Hayward kiwifruits on contents of L-ascorbic acid. J Food Agric Environ. 2010; 8(2): 132-4
  • 2. Mussel RL, De Sa Silva E, Costa AM, Mandarim-De-Lacerda CA. Mast cells in tissue response to dentistry materials: an adhesive resin, a calcium hydroxide and a glass ionomer cement. J Cell Mol Med. 2003; 7(2): 171-8[PubMed]
  • 3. Panahi MSS. Structure and Classification of Vascular Plants. 1998;
  • 4. Adzick NS. Wound healing:Textbook of surgery, The biological basis of modern surgical practic. 1997; : 576-640
  • 5. Khaksari M, Rezvani ME, Sajadi MA, Soleimani A. The effect of topically applied water extract of Rhazyastricta on cutaneous wound healing in rats. J Semnan Univ Med Sci. 2000; 1(3): 1-10
  • 6. Isler H, Bauen A, Hubler M, Oberholzer M. Morphometric assessment of wound healing in rats treated with a protein-free haemodialysate. Burns. 1991; 17(2): 99-103[PubMed]
  • 7. Taghizadeh-Jahed M, Jarolmasjed SH, Mohamadnejad S, Rezaii A, Delazar A. The effect of Echinacea purpurea aerial organ dried extract vs. Zinc oxide on skin wound healing in rat: a morphometric & histopathologic study. Tehran Univ Med Sci. 2008; 66(9): 625-32
  • 8. Lazareva EB, Smirnov SV, Khvatov VB, Spiridonova TG, Bitkova EE, Shramko LU, et al. [Oral administration of pectins for prophylaxis and treatment of purulent septic complications in patients with burns]. Antibiot Khimioter. 2002; 47(4): 16-9[PubMed]
  • 9. Sylvester KG, Nesbit M, Radu A, Herlyn M, Adzick NS, Crombleholme TM. Adenoviral-mediated gene transfer in wound healing: acute inflammatory response in human skin in the SCID mouse model. Wound Repair Regen. 2000; 8(1): 36-44[PubMed]
  • 10. Sobiczewska E, Szmigielski S. [The role of selected cell growth factors in the wound healing process]. Przegl Lek. 1997; 54(9): 634-8[PubMed]
  • 11. Check JH. Value of sperm morphology. Fertile Sterile. 1995; 8: 571-6
  • 12. Macleod J, Gold RZ. The male factor in fertility and infertility. VI. Semen quality and certain other factors in relation to ease of conception. Fertil Steril. 1953; 4(1): 10-33[PubMed]
  • 13. Esmael zadeh S, Farsi M, Bijany A. Effects of sperm morphology on pregnancy rate in IUI cycles. J Reprod Infertil. 2007; 8: 205-12
  • 14. Ollero M, Gil-Guzman E, Lopez MC, Sharma RK, Agarwal A, Larson K, et al. Characterization of subsets of human spermatozoa at different stages of maturation: implications in the diagnosis and treatment of male infertility. Hum Reprod. 2001; 16(9): 1912-21[PubMed]
  • 15. Turk G, Sonmez M, Aydin M, Yuce A, Gur S, Yuksel M, et al. Effects of pomegranate juice consumption on sperm quality, spermatogenic cell density, antioxidant activity and testosterone level in male rats. Clin Nutr. 2008; 27(2): 289-96[DOI][PubMed]
  • 16. Afaq F, Saleem M, Krueger CG, Reed JD, Mukhtar H. Anthocyanin- and hydrolyzable tannin-rich pomegranate fruit extract modulates MAPK and NF-kappaB pathways and inhibits skin tumorigenesis in CD-1 mice. Int J Cancer. 2005; 113(3): 423-33[DOI][PubMed]
  • 17. Seeram NP, Adams LS, Henning SM, Niu Y, Zhang Y, Nair MG, et al. In vitro antiproliferative, apoptotic and antioxidant activities of punicalagin, ellagic acid and a total pomegranate tannin extract are enhanced in combination with other polyphenols as found in pomegranate juice. J Nutr Biochem. 2005; 16(6): 360-7[DOI][PubMed]
  • 18. Sumner MD, Elliott-Eller M, Weidner G, Daubenmier JJ, Chew MH, Marlin R, et al. Effects of pomegranate juice consumption on myocardial perfusion in patients with coronary heart disease. Am J Cardiol. 2005; 96(6): 810-4[DOI][PubMed]
  • 19. Amin AR, Kucuk O, Khuri FR, Shin DM. Perspectives for cancer prevention with natural compounds. J Clin Oncol. 2009; 27(16): 2712-25[DOI][PubMed]
  • 20. Shariat S. Selection of medicinal plants. 2007;
  • 21. Zargari A. medicinal plants. 1992;
  • 22. Miyazawa M, Okuno Y, Nakamura S, Kosaka H. Antimutagenic activity of flavonoids from Pogostemon cablin. J Agric Food Chem. 2000; 48(3): 642-7[PubMed]
  • 23. Tutin TG, Hain T. Health benefits of cabbage. 2006; : 12-32
  • 24. Duke JA. Handbook of Medicinal Herbs, Second Edition. 2002; : 51-70
  • 25. Facciola S. Cornucopia: a source book of edible plants. 1990; : 45-52
  • 26. Parmar C, Kaushal MK. Wild Fruits of the Sub-Himalayan Region. 1982; : 25-39
  • 27. Kunkel G. Plants for human consumption: an annotated checklist of the edible phanerogams and ferns. 1984; : 152-63
  • 28. Koppikar SP. Clinical Experiences of 70 Years in Homoeopathy. 2002; : 12-32
  • 29. Gupta R, Manchanda RK. Textbook of Dermatology for Homoeopaths. 2005; : 61-79
  • 30. effect of plumbago Europaea plant on wounds and comparison with Dkspantynvl and phenytoin Iran Congress of Physiology and Pharmacology
  • 31. Amin GR. Popular Medicinal Plants of Iran. 1991; : 44-64
  • 32. All about plantain herb weed Plantago major article research.
  • 33. Zargari A. medicinal plants. 1997; 4
  • 34. Holetz FB, Pessini GL, Sanches NR, Cortez DA, Nakamura CV, Filho BP. Screening of some plants used in the Brazilian folk medicine for the treatment of infectious diseases. Mem Inst Oswaldo Cruz. 2002; 97(7): 1027-31[PubMed]
  • 35. Bayat M. Effect of topical twice daily application of honey on mast cells a full thickness skin wounds of rat. J Dermatol. 2008; 3(2): 23-6
  • 36. Kasaie M, Rashidipoor A. Effect of fish oil on topical burn edema in rats. Journal of Science Department of Health Semnan University of medical Sciences. 1996; 1: 3-15
  • 37. Modaghegh S, Salehian B, Tavassoli M, Djamshidi A, Rezai AS. Use of phenytoin in healing of war and non-war wounds. A pilot study of 25 cases. Int J Dermatol. 1989; 28(5): 347-50[PubMed]
  • 38. Barnes J, Anderson LA, Phillipson JD. St John's wort (Hypericum perforatum L.): a review of its chemistry, pharmacology and clinical properties. J Pharm Pharmacol. 2001; 53(5): 583-600[PubMed]
  • 39. Mamouri M. Refined fish oil for food and pharmaceutical applications. J Constr Res. 1993; 5(19): 27-31
  • 40. Bahraini tosy M, Fatehi S, Yazdi AS, Abbasid H. Comparison of laser: Neon on wound healing in diabetic rats and healthy. Iranian Journal of Medical Physics. 2005; 2(6): 23-32
  • 41. Teng CM, Lee LG, Ko FN, Huang TF. Inhibition of Platelet-Aggregation by Apigenin from Apium-Graveolens. Asia Pacific J Pharmacol. 1988; 3(2): 85-9
  • 42. Cardoso CR, Favoreto S, Jr, Oliveira LL, Vancim JO, Barban GB, Ferraz DB, et al. Oleic acid modulation of the immune response in wound healing: a new approach for skin repair. Immunobiology. 2011; 216(3): 409-15[DOI][PubMed]
  • 43. Johnston DE. Wound healing in skin. Vet Clin North Am Small Anim Pract. 1990; 20(1): 1-25[PubMed]
  • 44. Savunen TJ, Viljanto JA. Prediction of wound tensile strength: an experimental study. Br J Surg. 1992; 79(5): 401-3[PubMed]
  • 45. Brunicardi FC, Schwartz SI. Schwartz ́s principles of surgery 8 Ed. 2005;
  • 46. Janis JE, Kwon RK, Lalonde DH. A practical guide to wound healing. Plast Reconstr Surg. 2010; 125(6): 230-44[DOI][PubMed]
  • 47. Weinstein-Oppenheimer CR, Aceituno AR, Brown DI, Acevedo C, Ceriani R, Fuentes MA, et al. The effect of an autologous cellular gel-matrix integrated implant system on wound healing. J Transl Med. 2010; 8: 59[DOI][PubMed]
  • 48. Hadipur Jahrom M.. study on effect of Punica granatum extracts of experimental model of osteoarthritis in the knee joint cartilage protection. Med Sci J Islamic Azad Univ. 2007; 17(4)
  • 49. Toi D, Suratmiller M. Potential anti-angiogenic Punica granatum. J Agri Food Chem. 2003; 1(6): 44-59
  • 50. Soaring B. Plantago major/lanceolate. 2001; 1(1): 21-73
  • 51. Taghavi M, Khaksari M. Chronic skin wound healing in diabetic rats by topical fish oil. Acad J Semnan Univ Med Sci. 2002; 4(1, 2): 61-73
  • 52. Hasanzadeh G, Mehdikhanloo NS. The Effect of Compound of Brassica Oleracea L.and Egg-white on burn Wound Healing in Rat.carborough J. On medications for burns in classical antiquity. Clin Plast Surg. 1983; 10(4): 10-603
  • 53. Isbir T, Yaylim I, Aydin M, Ozturk O, Koyuncu H, Zeybek U, et al. The effects of Brassica oleraceae var capitata on epidermal glutathione and lipid peroxides in DMBA-initiated-TPA-promoted mice. Anticancer Res. 2000; 20(1A): 219-24[PubMed]
  • 54. Srivastava B, Shukla Y. Antitumour promoting activity of indole-3-carbinol in mouse skin carcinogenesis. Cancer Lett. 1998; 134(1): 91-5[PubMed]
  • 55. Dill RE, Iacopino AM. Myofibroblasts in phenytoin-induced hyperplastic connective tissue in the rat and in human gingival overgrowth. J Periodontol. 1997; 68(4): 375-80[DOI][PubMed]
  • 56. Song S, Cheng T. [The effect of systemic and local irradiation on wound macrophages and the repair promoting action of phenytoin sodium]. Zhonghua Yi Xue Za Zhi. 1997; 77(1): 54-7[PubMed]
  • 57. Byl NN, McKenzie AL, West JM, Whitney JD, Hunt TK, Scheuenstuhl HA. Low-dose ultrasound effects on wound healing: a controlled study with Yucatan pigs. Arch Phys Med Rehabil. 1992; 73(7): 656-64[PubMed]
  • 58. Riahy S, Imany H, Khoshbaten A. Effect of topical application of phenytoin and honey in closure of open wound in male rats. JAUMS. 2009; 7(2): 54-7
  • 59. Oluwatosin OM, Olabanji JK, Oluwatosin OA, Tijani LA, Onyechi HU. A comparison of topical honey and phenytoin in the treatment of chronic leg ulcers. Afr J Med Med Sci. 2000; 29(1): 31-4[PubMed]
  • 60. Lodha SC, Lohiya ML, Vyas MC, Bhandari S, Goyal RR, Harsh MK. Role of phenytoin in healing of large abscess cavities. Br J Surg. 1991; 78(1): 105-8[PubMed]
Creative Commons License Except where otherwise noted, this work is licensed under Creative Commons Attribution Non Commercial 4.0 International License .

Search Relations:



Create Citiation Alert
via Google Reader

Readers' Comments